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Kinetics of Urea Hydrolysis

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Quantifying rates of urea hydrolysis and CaCO3 precipitation in natural consortia is essential to estimate the efficiency of the process in both field trials and laboratory experiments. Furthermore, S. pasteurii, a facultative anaerobe, has the potential to act as a foreign inoculum in anoxic environments if indigenous communities lack ureolytic activity. Thus, it is essential to test this organism’s ability to hydrolyse urea and precipitate CaCO3 when injected into an anoxic natural groundwater. Note that these experiments will be carried out under high Ca2+ and urea concentrations, as will be required to seal fractures in rocks.

Calcite (left) and vaterite (right) crystal formed by ureolysis-driven CaCO3 precipitation in batch experiments with S. pasteurii. Note that bacteria get embedded within the forming crystals (see calcite crystal).

 

 

 

Spherical calcite crystals formed by ureolysis-driven CaCO3 precipitation in batch experiments with indigenous groundwater communities (stimulated with Molasses).

Last Updated on Monday, 13 June 2011 20:20  

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